![]() ![]() Pixel sizes & measurements #Īs described before, the pixel size influences measurements in ImageJ – but the units don’t appear anywhere in the Results table.įurthermore, once a measurement is added to the Results table it is fixed: it won’t automatically update if the pixel sizes are changed later.įor that reason, you need to be careful to check pixel sizes before making any measurements and make sure it’s clear to you which units have been used.įig. If the pixel size information is available, this is given in calibrated units. The size of the image is given at the top of each image window. The voxel depth (where relevant) is often different. The pixel width and height are usually the same. 44 Image properties for the Fluorescent Cells sample, which do not include useful pixel size information. Otherwise, the pixel sizes are given in the units displayed nearby in the properties dialog.įig. If the pixel size information is unavailable, the pixel sizes are given as 1 pixel: not terribly informative. 43 Image properties for the Confocal Series sample, which include the pixel size. There is also an additional value, Voxel depth, which is only relevant for z-stacks this gives the spacing between z-slices.įig. These are provided separately as values for Pixel width and Pixel height.Ī voxel is the 3D analogue of a pixel a ‘volume pixel’. You can see the pixel size for an image in ImageJ under Image ‣ Properties…. This section will explore pixel sizes and dimensions in ImageJ.Īlong the way, we will see how to generate scale bars and fix images where the pixel size or dimensions are incorrect – at least whenever we know what the correct values are. append ( './././' ) from helpers import * import numpy as np from matplotlib import pyplot as plt Introduction # ![]()
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